Lung cancer is the most common malignant tumor in hunan,and smoking is the first cause.The lung carcinogenesis is related to gene mutation,so its important to study the cell reaction to smoking and tumor generation in gene level.
cDNA microarray was used to screen out smoking related lung cancer genes，and the result was checked by RT-PCR. Inhibitor and activator was used to control the gene expression. TG2 expression level were detected by Western-blot and enzyme activity test,and flow cytometry was used to analyze cell apoptosis ,cell proliferation and cell cycle in malignant transformation of bronchial epithelial cells induced by cigarette smoking.
The total of differential expressed gene is 124,79 was up-regulated and 45 was down-regulated.These differential expressed can be divided into 27 kinds of group such as metabolism,cell apoptosis,cell cycle, Notch signal pathway,p53 signal pathway, peroxisome Proliferator-activated receptor pathway. The coincidence rate of RT-PCR and Gene chip was above 80%,which suggest the reliability of the gene chip result.
TG2 expression was down regulated in malignant transformed bronchial epithelial cells and cells exposed to cigarette smoking.TG2 expression and activitie change affect cell apoptosis,cell prolifiction and cell cycle.TG2 may affect cell malignant transformation through the regulation of these events.
Key words：lung cacer; gene chip; differential expressed gene; cigarette smoking; malignant transformation